CRISPR/Cas9介导的绵羊示踪脐带间充质干细胞系的建立
李松美(
),仇雨歌,陈胜男,王晓萌,王春生()
),仇雨歌,陈胜男,王晓萌,王春生()
CRISPR/Cas9 Mediated Exogenous Gene Knock-in at ROSA26 Locus in Sheep Umbilical Cord Mesenchymal Stem Cells
LI SongMei(),QIU YuGe,CHEN ShengNan,WANG XiaoMeng,WANG ChunSheng()
),QIU YuGe,CHEN ShengNan,WANG XiaoMeng,WANG ChunSheng()
图14. 阳性细胞克隆筛选
A：嘌呤霉素筛选16d后sUMSC-1（明视野50×）;B：嘌呤霉素筛选16d后sUMSC（暗视野50×）
（在sUMSCs细胞系中转入带有GFP的sROSA26-HA载体,转染24h后加压筛选16天后获得的细胞多数表达绿色荧光。之后,换为普通培养基）
Fig. 14. Cloning and screening of positive cells
A: After puromycin screening for 16d sUMSC-1（Bright field 50×）; B: After puromycin screening for 16d sUMSC（Dark field 50×）
(Transfect the sROSA26-HA vector with GFP into the sUMSCs cell line, add puromycin after transfecion 24h, most cells express green fluorescence after 16 days of screening.After that, change to normal medium.)
