CRISPR/Cas9介导的绵羊示踪脐带间充质干细胞系的建立
李松美(
),仇雨歌,陈胜男,王晓萌,王春生()
),仇雨歌,陈胜男,王晓萌,王春生()
CRISPR/Cas9 Mediated Exogenous Gene Knock-in at ROSA26 Locus in Sheep Umbilical Cord Mesenchymal Stem Cells
LI SongMei(),QIU YuGe,CHEN ShengNan,WANG XiaoMeng,WANG ChunSheng()
),QIU YuGe,CHEN ShengNan,WANG XiaoMeng,WANG ChunSheng()
图12. 筛选最佳嘌呤霉素浓度
A：sUMSCs（50×）;B：1.5μg·mL-1 嘌呤霉素筛选48h后sUMSCs（50×）;C：嘌呤霉素筛选结束时sUMSCs（50×）
（在汇合度为70%-80%的sUMSCs中加入抗性,记录细胞几乎全部死亡的时间和浓度作为后续抗性筛选依据）
Fig. 12. Select the best puromycin concentration
A: sUMSCs（50×）; B: After 1.5μg·mL-1 puromycin screening for 48h sUMSCs（50×）; C: At the end of puromycin screening sUMSCs（50×）
(Add puromycin to sUMSCs when the confluence was 70%-80%, and record the time and concentration of almost all cell death as the foundation for subsequent resistance screening. )
