基于简化基因组测序的越橘杂交后代鉴定

刘有春,刘威生,王兴东,孙斌,刘修丽,杨艳敏,魏鑫,杨玉春,张舵,刘成,李天忠

Identification of F1 Hybrids in Blueberry (Vaccinium corymbosum L.) Based on Specific-Locus Amplified Fragment Sequencing (SLAF-seq)

LIU YouChun,LIU WeiSheng,WANG XingDong,SUN Bin,LIU XiuLi,YANG YanMin,WEI Xin,YANG YuChun,ZHANG Duo,LIU Cheng,LI TianZhong

表1 越橘样品测序数据统计表

Table 1 Summary statistics of sequencing results from Vaccinium samples

样品名 Sample ID 总测序条数 Total reads 总碱基数量 Total bases 高质量reads百分比 Q30 percentage (%) GC含量百分比 GC percentage (%) Berkeley 10 350 711 2 067 990 444 95.12 39.14 N6 10 914 992 2 180 687 340 94.88 39.45 杂交后代 Offspring 971 057 193 850 201 95.04 39.72 水稻（对照样品） Rice (Control) 1 047 399 209 302 386 94.59 40.46 总和 Total 330 061 690 65 893 041 734 95.04 39.72