GA3介导miR171s及其靶基因VvSCLs调控葡萄种子发育的作用分析

王文然,解振强,诸葛雅贤,白云赫,管乐,吴伟民,张培安,郑婷,房经贵,王晨

Function Analysis of GA3 Mediate miR171s and Its Target Genes VvSCLs in Grape Seed Development

WANG WenRan,XIE ZhenQiang,ZHUGE YaXian,BAI YunHe,GUAN Le,WU WeiMin,ZHANG PeiAn,ZHENG Ting,FANG JingGui,WANG Chen

表1 vvi-miR171s预测靶基因结果

Table 1 vvi-miR171s target genes prediction results

miRNA成员 miRNA_Acc. miRNA 成熟体序列 miRNA_mature sequence 靶基因基因编号 Target_Acc. 期望值 Expectation 未配对时 的能量UPE 裂解区域 Target region 抑制作用 Inhibition 多重性 Multiplicity vvi-miR171a TGATTGAGCCGTGC CAATATC VIT_204s0023g01380.1(VvSCL15) 0.5 -1 CDS:656-676 裂解 Cleavage 1 VIT_215s0048g00270.1 (VvSCL6) 0.5 -1 CDS:1594-1614 裂解 Cleavage 1 VIT_202s0154g00400.1 (VvSCL22) 0.5 -1 CDS:1724-1744 裂解 Cleavage 1 vvi-miR171b TGATTGAGCCGCGT CAATATC VIT_204s0023g01380.1 (VvSCL15) 0.5 -1 CDS:656-676 裂解 Cleavage 1 VIT_215s0048g00270.1 (VvSCL6) 0.5 -1 CDS:1594-1614 裂解 Cleavage 1 VIT_202s0154g00400.1 (VvSCL22) 0.5 -1 CDS:1724-1744 裂解 Cleavage 1 vvi-miR171e TGATTGAGCCGCGC CAATATC VIT_204s0023g01380.1 (VvSCL15) 0 -1 CDS:656-676 裂解 Cleavage 1 VIT_215s0048g00270.1 (VvSCL6) 0 -1 CDS:1594-1614 裂解 Cleavage 1 VIT_202s0154g00400.1 (VvSCL22) 0 -1 CDS:1724-1744 裂解 Cleavage 1 vvi-miR171f TTGAGCCGCGCCAA TATCACT VIT_215s0048g00270.1 (VvSCL6) 1 -1 CDS:1591-1611 裂解 Cleavage 1 VIT_202s0154g00400.1 (VvSCL22) 1 -1 CDS:1721-1741 裂解 Cleavage 1 VIT_204s0023g01380.1 (VvSCL15) 1 -1 CDS:653-673 裂解 Cleavage 1 vvi-miR171g TTGAGCCGAACCAA TATCACC VIT_204s0023g01380.1 (VvSCL15) 4 -1 CDS:653-673 翻译 Translation 1 VIT_215s0048g00270.1 (VvSCL6) 4 -1 CDS:1591-1611 翻译 Translation 1 VIT_202s0154g00400.1 (VvSCL22) 4 -1 CDS:1721-1741 翻译 Translation 1 vvi-miR171h TGGTTGAGCCGCGC CAATATC VIT_204s0023g01380.1 (VvSCL15) 0.5 -1 CDS:656-676 裂解 Cleavage 1 VIT_215s0048g00270.1 (VvSCL6) 0.5 -1 CDS:1594-1614 裂解 Cleavage 1 VIT_202s0154g00400.1 (VvSCL22) 0.5 -1 CDS:1724-1744 裂解 Cleavage 1