图4. 无核白遗传转化过程（A）以及转基因植株的鉴定（B）
A：无核白遗传转化过程The genetic transformation of Thompson Seedless,a：无核白分生愈伤组织Meristem callus;b：侵染后的愈伤组织与农杆菌共培养The infected callus was co-cultured with A. tumefaciens;c：转化后的愈伤组织筛选培养Callus screening culture after transformation;d：筛选60 d后的愈伤组织Callus screening culture after 60 days;e：愈伤组织诱导出抗性芽Callus induced resistant buds;f：抗性芽生根培养Rooting culture of resistant bud;g：抗性芽长成植株Resistant buds grow into plants;h：抗性植株的继代扩繁Secondary propagation of resistant plants;i：继代后长成植株Plant growth after succession;j：转基因植株移栽Transplanting of transgenic plants。B：转基因植株的鉴定Identification of transgenic plants,a：转基因植株Transgenic plants;b：PCR检测抗性植株PCR was used to detect resistant plants,M为Marker,P为质粒M was Marker, and P was plasmid;c：Western blot检测抗性植株Western blot was used to detect resistant plants

Fig. 4. The genetic transformation of Thompson Seedless (A) and identification of transgenic plants (B)