图3. VqSTS9、VqSTS21基因克隆与植物表达载体构建
A：两个表达载体示意图Schematic diagram of two expression vectors。B：VqSTS9、VqSTS21基因连接表达载体pCAMBIA2300的双酶切检测VqSTS9, VqSTS21 genetic connection expression vector pCAMBIA2300 double enzyme detection,M：DNA Marker;1：BamH Ι单酶切表达载体质粒The BamH Ι single enzyme expression vector plasmid;2：Sal Ι单酶切表达载体质粒The Sal Ι single enzyme expression vector plasmid;P：pCAMBIA2300的空载体质粒对照Empty vector pCAMBIA2300 as control;CK：空白对照Blank control (ddH₂O);3—5：BamH Ι和Sal Ι双酶切植物表达载体 pCAMBIA35S::VqSTS9::GFP,3个重复Double enzyme of BamH Ι and Sal Ι plant expression vector pCAMBIA35S: :VqSTS9: :GFP, three repeats;6—8：BamH Ι和Sal Ι双酶切植物表达载体pCAMBIA35S::VqSTS21::GFP,3个重复Double enzyme of BamH Ι and Sal Ι plant expression vector pCAMBIA35S::VqSTS21::GFP, three repeats。C：过表达载体转化农杆菌的菌液PCR检测PCR detection of Agrobacterium-transformed by overexpression vector,M：DNA Marker;P：阳性质粒对照Positive plasmid control;1—3：农杆菌转化pCAMBIA35S::VqSTS9::GFP载体,3个重复Agrobacterium-transformed pCAMBIA35S::VqSTS9::GFP vector, three repeats;4—6：农杆菌转化pCAMBIA35S::VqSTS21::GFP载体, 3个重复Agrobacterium-transformed pCAMBIA35S::VqSTS21::GFP vector, three repeats

Fig. 3. VqSTS9 and VqSTS21 gene cloning and plant expression vector construction