陆地棉盐胁迫应答基因GhPEAMT1的克隆及功能分析
王娜1(
),赵资博2,高琼1,何守朴1,马晨辉1,彭振1,2(),杜雄明1,2()
),赵资博2,高琼1,何守朴1,马晨辉1,彭振1,2(),杜雄明1,2()
Cloning and Functional Analysis of Salt Stress Response Gene GhPEAMT1 in Upland Cotton
WANG Na1(),ZHAO ZiBo2,GAO Qiong1,HE ShouPu1,MA ChenHui1,PENG Zhen1,2(),DU XiongMing1,2()
),ZHAO ZiBo2,GAO Qiong1,HE ShouPu1,MA ChenHui1,PENG Zhen1,2(),DU XiongMing1,2()
图8. GhPEAMT1沉默效率和盐处理后的表型
A：沉默标记基因TRV2:CLA植株;B：病毒诱导基因沉默后,GhPEAMT1A/D沉默效率分析,1—7：注射TRV2::GhPEAMT1A、TRV2::GhPEAMT1D菌株的株系;C—D：盐胁迫48 h后,CK、TRV2:00、TRV2::GhPEAMT1A、TRV2::GhPEAMT1D棉花幼株表型
Fig. 8. GhPEAMT1 gene silencing efficiency and phenotype after salt treatment
A: Silence marker gene TRV2: CLA plants; B: Analysis of GhPEAMT1 gene silencing efficiency after virus-induced gene silencing, abscissas 1-7: Strains injected with TRV2::GHPEAMT1A, TRV2::GhPEAMT1D strains; C-D: 48 h after salt stress, CK, TRV2:00, TRV2::GHPEAMT1A, TRV2::GHPEAMT1D phenotype of cotton young plants
