陆地棉盐胁迫应答基因GhPEAMT1的克隆及功能分析

王娜,赵资博,高琼,何守朴,马晨辉,彭振,杜雄明

Cloning and Functional Analysis of Salt Stress Response Gene GhPEAMT1 in Upland Cotton

WANG Na,ZHAO ZiBo,GAO Qiong,HE ShouPu,MA ChenHui,PENG Zhen,DU XiongMing

表1 研究中使用的引物

Table 1 Primers used in the study

引物名称Primer name 正向引物Forward primer (5′-3′) 反向引物Reverse primer (5′-3′) GhActin ATCCTCCGTCTTGACCTTG TGTCCGTCAGGCAACTCAT GhPEAMT1A GCTCTAGAATGGCGGCTAACGGATTT CCGGGATCCTTAATTCTTCTTCTTCTTGGC GhPEAMT1D GCTCTAGAATGGCGGCTAACGGATTT CGGGATCCTTAGTTCTTCTTCTTGGC qPEAMT1A GAGCACAGGAGGAATTGCAG TTACAGAGAGGTCGTTGCCC qPEAMT1D GAGCACAGGAGGAATTGCAG GGTCGATGCCCACAACATGA CPEAMT1A ACGCGTCGACGCGGCTAACGGATTTGTA GTCGACTTAATTCTTCTTCTTCTTGGC CPEAMT1D ACGCGTCGACGCGGCTAACGGATTTGTAGG GTCGACTTAGTTCTTCTTCTTGGCAAGG VPEAMT1A GCTCTAGAATGGCGGCTAACGGATTTGT CGGGATCCATAATGCCCATTGATGGTTT VPEAMT1D GCTCTAGATCTTGCTTTCATCAATCTGG CGGGATCCAAAGACACGCTCATAACG