小麦籽粒黄色素含量检测方法的改良与应用

翟胜男,刘爱峰,李法计,刘成,郭军,韩冉,訾妍,汪晓璐,吕莹莹,刘建军

Improvement and Application of the Method for Determining Yellow Pigment Content in Wheat Grain

ZHAI ShengNan,LIU AiFeng,LI FaJi,LIU Cheng,GUO Jun,HAN Ran,ZI Yan,WANG XiaoLu,LÜ YingYing,LIU JianJun

表2 2种黄色素含量测定方法比较

Table 2 Comparison of two methods for detecting yellow pigment content

项目Item 传统AACC 14-50法 AACC 14-50 method 改良方法 Modified method 面粉用量 Flour consumption (g) 8.0 0.5 水饱和正丁醇用量 Amount of n-butanol saturated with water (mL) 40 2.5 检测方法 Detection method 分光光度计 Spectrophotometer 酶标仪 Multiskan spectrum microplate reader 上样容器 Container 比色皿 Cuvette 96孔酶标板 96-well microtiter plate 上样量 Loading quantity 1 mL 200 μL 检测效率 Detection efficiency 一次只能检测单个样本,1个样品/分钟 Only one sample can be tested at a time, one sample/min 同时检测多个样品,12—15个样品/分钟 Multiple samples can be tested simultaneously, 12-15 samples/min