猪I型补体受体与C3b活性片段相互结合的体外检测

孙雨晨,贾瑞璞,范阔海,孙娜,孙耀贵,孙盼盼,李宏全,尹伟

Detection of Interaction Between Porcine Type I Complement Receptor and C3b Active Fragment in Vitro

SUN YuChen,JIA RuiPu,FAN KuoHai,SUN Na,SUN YaoGui,SUN PanPan,LI HongQuan,YIN Wei

表3 配制10%分离胶

Table 3 Preparation of 10% separation gel

试剂 Reagents 体积 Volumes H2O 3.20 mL 30% Acr-Bis（29:1） 2.64 mL 1.5 mol·L-1 Tris-HCl（pH8.8） 2.00 mL 10% SDS 0.08 mL 10% AP 0.08 mL N,N,N',N'-四甲基乙二胺TEMED 3.00 µL