猪I型补体受体与C3b活性片段相互结合的体外检测

孙雨晨,贾瑞璞,范阔海,孙娜,孙耀贵,孙盼盼,李宏全,尹伟

Detection of Interaction Between Porcine Type I Complement Receptor and C3b Active Fragment in Vitro

SUN YuChen,JIA RuiPu,FAN KuoHai,SUN Na,SUN YaoGui,SUN PanPan,LI HongQuan,YIN Wei

表2 配制5%浓缩胶

Table 2 Preparation of 5% concentrated gel

试剂 Reagents 体积 Volumes H2O 2.67 mL 30% Acr-Bis（29:1） 0.67 mL 1.0 mol·L-1 Tris-HCl（pH6.8） 0.67 mL 10% SDS 53.3 µL 10% AP 40.0 µL N,N,N',N'-四甲基乙二胺TEMED 5.33 µL