静原鸡肌肉组织肌苷酸特异性沉积相关LNC_003828- gga-miR-107-3p-MINPP1的关联分析

禹保军,邓占钊,辛国省,蔡正云,顾亚玲,张娟

Correlation Analysis of Inosine Monophosphate Specific Deposition Related LNC_003828-gga-miR-107-3P-MINPP1 in Jingyuan Chicken Muscle Tissue

YU BaoJun,DENG ZhanZhao,XIN GuoSheng,CAI ZhengYun,GU YaLing,ZHANG Juan

表5 荧光定量PCR体系

Table 5 Fluorescence quantitative PCR system

成分 Components 终浓度 Final concentration 加样量 Loading volume (μL) 2×SYBR® Green Supermix 1× 5 Forward primer 200 nmol·L-1 0.5 Reverse primer 200 nmol·L-1 0.5 cDNA N/A 1 ddH2O N/A 3