二化螟P糖蛋白基因的克隆分析及对杀虫剂的诱导响应

孟祥坤,吴赵露,杨雪梅,官道杰,王建军

Cloning and Analysis of P-glycoprotein Gene and Its Transcriptional Response to Insecticide in Chilo suppressalis

MENG XiangKun,WU ZhaoLu,YANG XueMei,GUAN DaoJie,WANG JianJun

表1 用于CsPgp克隆与分析的引物序列

Table 1 The primers used for gene cloning and analysis of CsPgp

引物名称Primer name 序列Sequence (5′ to 3′) 作用Function CsPgp F CAGCTTTGAATCAGGACTTTGC CsPgp基因片段扩增 Gene fragment amplification of CsPgp CsPgp R ATCCACTCCGATTGAGGTTGTA CsPgp 5′R1 CCATCCCTTCACAAGTGCCATTAT CsPgp 5′端片段扩增 5′ end fragment amplification of CsPgp CsPgp 5′R2 CGAACGCCGATTGGTAGAAAAC CsPgp 3′F1 GCGTTGGATACGGAAAGCGAAAA CsPgp 3′端片段扩增 3′ end fragment amplification of CsPgp CsPgp 3′F2 AGTGCGGGACGCCGACCTTATA DLCsPgp F GCAGGATGCACCACTCCTATCA CsPgp定量分析 Quantitative analysis of CsPgp DLCsPgp R TGGCCGCTCCGACTATACAGTTA EF-1α F TGAACCCCCATACAGCGAATCC EF-1α R TCTCCGTGCCAACCAGAAATAGG CsPgp 5′F GCACTATTACACTTTTTAATGAG CsPgp 5′侧翼区DNA序列克隆 5′ lateral DNA sequence cloning of CsPgp CsPgp 5′R GCGTTTCCGTTCAAATCATAAG