本氏烟NbNAC062的克隆及对马铃薯Y病毒侵染的抑制作用

曲潇玲,焦裕冰,罗健达,宋丽云,李莹,申莉莉,杨金广,王凤龙

Cloning of Nicotiana benthamiana NAC062 and Its Inhibitory Effect on Potato Virus Y Infection

QU XiaoLing,JIAO YuBing,LUO JianDa,SONG LiYun,LI Ying,SHEN LilLi,YANG JinGuang,WANG FengLong

图7 过表达NbNAC062后PVY与BiP积累量变化 A：过表达NbNAC062后PVY侵染1、3、5、7 d,qRT-PCR检测PVY CP mRNA变化Changes of PVY CP mRNA are detected by qRT-PCR when PVY infection was 1, 3, 5, 7 days after overexpression NbNAC062;B：过表达NbNAC062后PVY侵染1、3、5、7 d,qRT-PCR检测BiP变化Changes of BiP are detected by qRT-PCR when PVY infection was 1, 3, 5, 7 days after overexpression NbNAC062;C：过表达NbNAC062后PVY侵染1、3、5、7 d,Western blot检测PVY蛋白量变化。每天取样组中左侧为对照右侧为处理Changes of PVY protein are detected by Western blot when PVY infection was 1, 3, 5, 7 days after overexpression NbNAC062. In the daily sampling group, the left side is the control and the right side is the treatment;D：C对应的Actin Western blot蛋白杂交图The Western blot protein hybridization map of Actin corresponding to C

Fig. 7 Changes in PVY and BiP accumulation after overexpression of NbNAC062