本氏烟NbNAC062的克隆及对马铃薯Y病毒侵染的抑制作用
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曲潇玲,焦裕冰,罗健达,宋丽云,李莹,申莉莉,杨金广,王凤龙
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Cloning of Nicotiana benthamiana NAC062 and Its Inhibitory Effect on Potato Virus Y Infection
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QU XiaoLing,JIAO YuBing,LUO JianDa,SONG LiYun,LI Ying,SHEN LilLi,YANG JinGuang,WANG FengLong
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表1 本试验所用引物
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Table 1 Primers used in this study
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| 引物Primer | 序列Sequence | | NbNAC062 F | ATGATGGCAGTACTTCCTGG | | NbNAC062 R | TACTCGCACTCTAAAGTATTCCC | | TRV-NbNAC F | TAAGGTTACCGAATTCTTGGATGGATCACACCCTGGC | | TRV-NbNAC R | AGACGCGTGAGCTCGGTACCTTCTGTATCATCAGCAATACAGC | | Fu-NbNAC F | CTTTAGATCTTCTAGAATGATGGCAGTACTTCCTGG | | Fu-NbNAC R | AGGAGGCCATGAATTCTACTCGCACTCTAAAGTATTCCC | | PVY-CP-F | GATGAATGGGCTTATGGTTTGGTG | | PVY-CP-R | GATTTGCCTAAGGGTTGGTTTCG | | Actin-F | CAAGGAAATCACCGCTTTGG | | Actin-R | AAGGGATGCGAGGATGGA | | qPCR-NbNAC062 F | TGGACAAGAATTGGCATCGC | | qPCR-NbNAC062 R | AACACCTCGGGCTCAAAGAAG | | qPCR-BiP F | GCCACAGAAGAAGCTACCAAGTTG | | qPCR-BiP R | GGTCCTCTCTGGGTTAACAGCG |
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