基于SLAF-seq技术鉴定苹果砧木耐涝候选基因

宋春晖,陈晓菲,王枚阁,郑先波,宋尚伟,焦健,王苗苗,马锋旺,白团辉

Identification of Candidate Genes for Waterlogging Tolerance in Apple Rootstock by Using SLAF-seq Technique

SONG ChunHui,CHEN XiaoFei,WANG MeiGe,ZHENG XianBo,SONG ShangWei,JIAO Jian,WANG MiaoMiao,MA FengWang,BAI TuanHui

表5 定位区间基因功能预测

Table 5 Functional annotation of gene mapping segments

编号No 基因编号Gene ID 注释信息 Function annotation 1 MD10G1014300 生长素输出载体家族蛋白 Auxin efflux carrier family protein 2 MD10G1014400 核小分子RNA71 SnoR71 3 MD10G1014500 乙醇脱氢酶1 Alcohol dehydrogenase 1 4 MD10G1014600 5S核糖体RNA 5S rRNA 5 MD10G1014700 5S核糖体RNA 5S rRNA 6 MD10G1014800 植物硬脂酰酰基载体蛋白去饱和酶家族蛋白 Plant stearoyl-acyl-carrier-protein desaturase family protein 7 MD10G1014900 管状分子分化相关6 Tracheary element differentiation-related 6 8 MD10G1015000 表皮毛双折射7 TRICHOME BIREFRINGENCE-LIKE 7 9 MD10G1015100 表皮毛双折射7 TRICHOME BIREFRINGENCE-LIKE 7 10 MD10G1015200 植物U3 Plant U3 11 MD10G1015300 抗病蛋白（TIR-NBS-LRR类） Disease resistance protein (TIR-NBS-LRR class) 12 MD10G1015400 非编码RNA ncRNA 13 MD10G1015500 丝氨酸羧肽酶42 Serine carboxypeptidase-like 42 14 MD10G1015600 三角状五肽重复序列（PPR）超家族蛋白 Pentatricopeptide repeat (PPR) superfamily protein 15 MD10G1015700 三角状五肽重复序列（PPR）超家族蛋白 Pentatricopeptide repeat (PPR) superfamily protein 16 MD10G1015800 植物糖原蛋白样淀粉起始蛋白2 Plant glycogenin-like starch initiation protein 2 17 MD10G1015900 植物糖原蛋白样淀粉起始蛋白2 Plant glycogenin-like starch initiation protein 2 18 MD10G1016000 液泡蛋白分选45 Vacuolar protein sorting 45 19 MD10G1016100 液泡蛋白分选45 Vacuolar protein sorting 45 20 MD10G1016200 5S核糖体RNA 5S rRNA 21 MD10G1016300 功能未知的蛋白（DUF506） Protein of unknown function (DUF506) 22 MD10G1016400 高尔基核苷酸糖转运蛋白1 Golgi nucleotide sugar transporter 1 23 MD10G1016500 ABC转运蛋白家族蛋白 ABC transporter family protein 24 MD10G1016600 功能未知的蛋白质（DUF300） Protein of unknown function (DUF300) 25 MD10G1016700 snoZ221 snoR21b 26 MD10G1016800 发病相关家族蛋白 Pathogenesis-related family protein 27 MD10G1016900 核糖体RNA 5S rRNA 5S 28 MD10G1017000 snoZ221 snoR21b 29 MD10G1017100 未知 Unknown 30 MD10G1017200 5S核糖体RNA 5S rRNA 31 MD10G1017300 发病相关家族蛋白 Pathogenesis-related family protein 32 MD10G1017400 糖脂转移蛋白2 Glycolipid transfer protein 2 33 MD10G1017500 未表征的蛋白质 Uncharacterized protein 34 MD10G1017600 真核生物翻译起始因子6-2 Eukaryotic translation initiation factor 6-2 35 MD10G1017700 功能未知的蛋白质 Protein of unknown function 36 MD10G1017800 Dof型锌指DNA结合家族蛋白 Dof-type zinc finger DNA-binding family protein 37 MD10G1017900 ABC转运G家族成员22 ABC transporter G family member 22-like 38 MD10G1018000 MAP激酶底物1 MAP kinase substrate 1 39 MD10G1018100 核内体靶向BRO1样结构域含蛋白 Endosomal targeting BRO1-like domain-containing protein 编号No 基因编号Gene ID 注释信息 Function annotation 40 MD10G1018200 RING / FYVE / PHD锌指超家族蛋白 RING/FYVE/PHD zinc finger superfamily protein 41 MD10G1018300 伴刀豆球蛋白A样凝集素蛋白激酶家族蛋白 Concanavalin A-like lectin protein kinase family protein 42 MD10G1018400 抗病蛋白（TIR-NBS-LRR类）家族 Disease resistance protein (TIR-NBS-LRR class) family 43 MD10G1018500 抗病蛋白（TIR-NBS-LRR类） Disease resistance protein (TIR-NBS-LRR class) 44 MD10G1018600 抗病蛋白（TIR-NBS-LRR类） Disease resistance protein (TIR-NBS-LRR class) 45 MD10G1018700 嘌呤生物合成4 Purine biosynthesis 4 46 MD10G1018800 未知 Unknown 47 MD10G1018900 抗病蛋白（TIR-NBS-LRR类） Disease resistance protein (TIR-NBS-LRR class) 48 MD10G1019000 未知 Unknown 49 MD10G1019100 铜转运蛋白2 Copper transporter 2-like 50 MD10G1019200 抗病蛋白（TIR-NBS-LRR类） Disease resistance protein (TIR-NBS-LRR class) 51 MD10G1019300 小核糖核蛋白家族蛋白 Small nuclear ribonucleoprotein family protein 52 MD10G1019400 伴刀豆球蛋白A样凝集素蛋白激酶家族蛋白 Concanavalin A-like lectin protein kinase family protein 53 MD10G1019500 伴刀豆球蛋白A样凝集素蛋白激酶家族蛋白 Concanavalin A-like lectin protein kinase family protein 54 MD10G1019600 伴刀豆球蛋白A样凝集素蛋白激酶家族蛋白 Concanavalin A-like lectin protein kinase family protein 55 MD10G1019700 抗病蛋白（TIR-NBS-LRR类）家族 Disease resistance protein (TIR-NBS-LRR class) family 56 MD10G1019800 伴刀豆球蛋白A样凝集素蛋白激酶家族蛋白 Concanavalin A-like lectin protein kinase family protein 57 MD10G1019900 伴刀豆球蛋白A样凝集素蛋白激酶家族蛋白 Concanavalin A-like lectin protein kinase family protein 58 MD10G1020000 抗病蛋白（TIR-NBS-LRR类） Disease resistance protein (TIR-NBS-LRR class) 59 MD10G1020100 抗病蛋白（TIR-NBS-LRR类） Disease resistance protein (TIR-NBS-LRR class) 60 MD10G1020200 未知 Unknown 61 MD10G1020300 伴刀豆球蛋白A样凝集素蛋白激酶家族蛋白 Concanavalin A-like lectin protein kinase family protein 62 MD10G1020400 含LIM结构域的蛋白质 LIM domain-containing protein 63 MD10G1020500 抗病蛋白（TIR-NBS-LRR类） Disease resistance protein (TIR-NBS-LRR class) 64 MD10G1020600 非编码RNA ncRNA 65 MD10G1020700 线粒体底物载体家族蛋白 Mitochondrial substrate carrier family protein 66 MD10G1020800 含LIM结构域的蛋白质 LIM domain-containing protein 67 MD10G1020900 抗病蛋白（TIR-NBS-LRR类） Disease resistance protein (TIR-NBS-LRR class) 68 MD10G1021000 抗病蛋白（TIR-NBS-LRR类） Disease resistance protein (TIR-NBS-LRR class) 69 MD10G1021100 功能未知的蛋白（DUF506） Protein of unknown function (DUF506) 70 MD10G1021200 β-酮酰基还原酶1 Beta-ketoacyl reductase 1 71 MD10G1021300 具有 FYVE 锌指结构域的染色体浓缩（RCC1）家族调节因子 Regulator of chromosome condensation (RCC1) family with FYVE zinc finger domain 72 MD10G1021400 植物U-box 29 Plant U-box 29 73 MD10G1021500 RNA结合蛋白2 RNA-binding protein 2-like 74 MD10G1021600 核苷酸结合 Nucleotide binding 75 MD10G1021700 未知 Unknown 76 MD10G1021800 非编码RNA ncRNA 77 MD10G1021900 ACT样蛋白酪氨酸激酶家族蛋白 ACT-like protein tyrosine kinase family protein 78 MD10G1022000 碳酸酐酶1 Carbonic anhydrase 1 79 MD10G1022100 F盒家族蛋白 F-box family protein 编号No 基因编号Gene ID 注释信息 Function annotation 80 MD10G1022200 碳酸酐酶2 Carbonic anhydrase 2 81 MD10G1022300 未知 Unknown 82 MD10G1022400 碳酸酐酶2 Carbonic anhydrase 2 83 MD10G1022500 RmlC样铜蛋白超家族蛋白 RmlC-like cupins superfamily protein 84 MD10G1022600 RmlC样铜蛋白超家族蛋白 RmlC-like cupins superfamily protein 85 MD10G1022700 未知 Unknown 86 MD10G1022800 未知 Unknown 87 MD10G1022900 RmlC样铜蛋白超家族蛋白 RmlC-like cupins superfamily protein 88 MD10G1023000 综合调控因子2 General regulatory factor 2 89 MD10G1023100 样铜蛋白超家族蛋白 RmlC-like cupins superfamily protein RmlC 90 MD10G1023200 TCP-1/cpn60伴侣蛋白家族蛋白 TCP-1/cpn60 chaperonin family protein 91 MD10G1023300 含LIM结构域的蛋白质 LIM domain-containing protein 92 MD10G1023400 泛素载体蛋白7 Ubiquitin carrier protein 7 93 MD10G1023500 胚芽蛋白样蛋白2 Germin-like protein 2 94 MD10G1023600 磷酸吡rid醛（PLP）依赖性转移酶 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein 95 MD10G1023700 5S 核糖体RNA 5S rRNA 96 MD10G1023800 VIRE2相互作用蛋白 1VIRE2-interacting protein 1 97 MD10G1023900 Tho复合亚基7 / Mft1p Tho complex subunit 7/Mft1p 98 MD10G1024000 液泡铁转运蛋白（VIT）家族蛋白 Vacuolar iron transporter (VIT) family protein 99 MD10G1024100 真核翻译起始因子2（eIF-2）家族蛋白 Eukaryotic translation initiation factor 2 (eIF-2) family protein 100 MD10G1024200 非编码RNA ncRNA 101 MD10G1024300 泛素相互作用基序蛋白 Ubiquitin interaction motif-containing protein 102 MD10G1024400 果糖-1,6-二磷酸酶 Fructose-1,6-bisphosphatase 103 MD10G1024500 小核糖核蛋白家族蛋白 Small nuclear ribonucleoprotein family protein 104 MD10G1024600 小核糖核蛋白家族蛋白 Small nuclear ribonucleoprotein family protein 105 MD10G1024700 U6 106 MD10G1024800 序列特异性DNA结合转录因子 Sequence-specific DNA binding transcription factors 107 MD10G1024900 非编码RNA ncRNA 108 MD10G1025000 未知 Unknown 109 MD10G1025100 Trihelix转录因子 Trihelix transcription factor 110 MD10G1025200 Trihelix转录因子 Trihelix transcription factor 111 MD10G1025300 结瘤素MtN21/EamA样转运蛋白家族蛋白 Nodulin MtN21/EamA-like transporter family protein 112 MD10G1025400 U6 113 MD10G1025500 DEAD-box ATP依赖性RNA解旋酶21 DEAD-box ATP-dependent RNA helicase 21-like 114 MD10G1025600 桶状样F-box蛋白8（LOC103444645）,mRNA Tubby-like F-box protein 8 (LOC103444645), mRNA 115 MD10G1025700 核小分子RNA118 snoR118 116 MD10G1025800 未知 Unknown 117 MD10G1025900 功能未知的蛋白质（DUF793） Protein of unknown function (DUF793) 118 MD10G1026000 非编码RNA ncRNA 119 MD10G1026100 HSP20样伴侣蛋白超家族蛋白 HSP20-like chaperones superfamily protein 120 MD10G1026200 铜氧还蛋白超家族蛋白 Cupredoxin superfamily protein