基于SLAF-seq技术鉴定苹果砧木耐涝候选基因

宋春晖,陈晓菲,王枚阁,郑先波,宋尚伟,焦健,王苗苗,马锋旺,白团辉

Identification of Candidate Genes for Waterlogging Tolerance in Apple Rootstock by Using SLAF-seq Technique

SONG ChunHui,CHEN XiaoFei,WANG MeiGe,ZHENG XianBo,SONG ShangWei,JIAO Jian,WANG MiaoMiao,MA FengWang,BAI TuanHui

表1 样品测序质量及与参考基因组的匹配情况

Table 1 Quality of sample sequencing and matching of the data with reference genome

样本 Sample 干净reads Clean reads 干净碱基 Clean base Q30 (%) GC (%) 总比对率 Total mapped (%) 正确比对率 Properly mapped (%) G41 38 365 575 9 666 919 570 85.02 41.1 97.55 87.64 新疆野苹果M. sieversii 39 983 972 10 067 312 448 87.82 41.61 97.48 85.77 耐涝混池 Tolerant pool 10 873 511 2 174 702 200 89.47 39.89 94.94 82.30 不耐涝混池 Sensitive pool 10 279 180 2 055 836 000 88.94 39.86 93.01 80.64