甜菜夜蛾双重氧化酶基因SeDuox的克隆、表达及功能分析

付超然,李亚子,吴涵,赵丹,郭巍,郭晓昌

Cloning, Expression and Functional Analysis of SeDuox from Spodoptera exigua

FU ChaoRan,LI YaZi,WU Han,ZHAO Dan,GUO Wei,GUO XiaoChang

图5 重组蛋白SeDuox-OM在昆虫细胞Sf9中的表达 M：λ-EcoT14 I digest;A-1：pFastBacTM HTA-SeDuox-OM重组质粒单酶切 (EcoR I) Enzyme analysis of pFastBacTM HTA-SeDuox-OM recombinant plasmid with EcoR I;A-2：pFastBacTM HTA-SeDuox-OM重组质粒双酶切 (EcoR I & BamH I) Enzyme analysis of pFastBacTM HTA-SeDuox-OM recombinant plasmid with EcoR I & BamH I;B-1：重组Bacmid DNA质粒Recombinant Bacmid DNA plasmid;C-M：蛋白预染Marker Protein predyeing Marker;C-1：未感染病毒的Sf9细胞上清The supernatant of Sf9 cells uninfected with virus;C-2：感染病毒的Sf9细胞上清The supernatant of Sf9 cells infected with virus

Fig. 5 Expression of recombinant protein SeDuox-OM in Sf9 cells