甜菜夜蛾双重氧化酶基因SeDuox的克隆、表达及功能分析

付超然,李亚子,吴涵,赵丹,郭巍,郭晓昌

Cloning, Expression and Functional Analysis of SeDuox from Spodoptera exigua

FU ChaoRan,LI YaZi,WU Han,ZHAO Dan,GUO Wei,GUO XiaoChang

图1 SeDuox编码氨基酸序列分析 黑色实线标注的为SeDuox蛋白的信号肽序列,长方形标注为跨膜区,绿色波浪线标注为过氧化物酶结构域,短虚线标注为N-端钙离子结合域,双虚线标注为铁还原酶结构域,双实线标注为黄素腺嘌呤二核苷酸结构域,长虚线标注为烟酰胺腺嘌呤二核苷酸结构域,红色箭头标注为O-糖基化位点,黑色箭头标注为N-糖基化位点

Fig. 1 Analysis of amino acid sequence of SeDuox The signal peptide sequence of SeDuox protein is marked with black solid line, the transmembrane region is labeled with rectangle, the peroxidase domain is labeled with green wavy line, the N-terminal calcium binding domain (EFH) is labeled with short dashed line, the iron reductase binding domain (Ferric) is labeled with double dashed line, the flavin adenie dinucleotide binding domain (FAD) is labeled with double solid line, the nicotinamide adenine dinucleotide binding domain (NAD) is labeled with long dashed line, the O-glycosylation site is marked with the red arrow, and the N-glycosylation site is marked with the black arrow