甜菜夜蛾双重氧化酶基因SeDuox的克隆、表达及功能分析
付超然,李亚子,吴涵,赵丹,郭巍,郭晓昌

Cloning, Expression and Functional Analysis of SeDuox from Spodoptera exigua
FU ChaoRan,LI YaZi,WU Han,ZHAO Dan,GUO Wei,GUO XiaoChang
表1 引物信息
Table 1 Primer information
引物名称
Primer name
引物序列
Primer sequence
SeDuox-F GGTTCAACAATAGAGCTTACCC
SeDuox-R TGACTTGCTCGCATGCGGACAT
SeDuox-OM-F CGGATCCGCCAGAAGTGTACTACGAGAAGC
SeDuox-OM-R CGGAATTCGTTGCCCTCGAAGTAGTCATAG
SeActin-F CTACCTCACGCCATTCTC
SeActin-R AACCTGAGTCTTTGTGTACCTCC
SeqPCR-F CGTATACTAATGGACCAGGTTTTCG
SeqPCR-R AACCTGAGTCTTTGTGTACCTCC
dsSeDuox-T7-F TAATACGACTCACTATAGGCCGCCATACTACTGA
GAGAC
dsSeDuox-T7-R TAATACGACTCACTATAGGGATGACCCGTGGATT
CTAAC
dsSeDuox-F CCGCCATACTACTGAGAGAC
dsSeDuox-R GATGACCCGTGGATTCTAAC
dsGFP-T7-F TAATACGACTCACTATAGGCCACAAGTTCAGCGT
GT
dsGFP-T7-R TAATACGACTCACTATAGGAGTTCACCTTGATGC
CG
dsGFP-F CCACAAGTTCAGCGTGT
dsGFP-R AGTTCACCTTGATGCCG
16S-F TCCTACGGGAGGCAGCAGT
16S-R GGACTACCAGGGTATCTAATCCTGTT
Attacin-F GGACTGACCGTGATGAAGG
Attacin-R CGTTGTGGTTGTCGTTGTG
Definsin-F GACTTGGACGCGAAATTGA
Definsin-R TGGTAGACAATGCTCCTGG
PGRP-F CACTCGAACTTGCAATACGG
PGRP-R ACCAATCACGAACGAGGACC
Es-F CCCTTATTGTTAGTTGCCATCATT
Es-R ACTCGTTGTACTTCCCATTGT
Bc-F GCCCTGGTATGTATATTGGATCTAC
Bc-R GGTCATAATAACTTCTACAGCAGGA
下划线分别表示酶切位点和T7 RNA聚合酶启动子
Restriction enzyme cutting site and T7 RNA polymerase promoter are underlined