转CiNPR4基因柑橘抗溃疡病的机制解析

张婧芸,刘语诺,王兆昊,彭爱红,陈善春,何永睿

Analysis of Resistance Mechanism of CiNPR4 Transgenic Plants to Citrus Canker

ZHANG JingYun,LIU YuNuo,WANG ZhaoHao,PENG AiHong,CHEN ShanChun,HE YongRui

图3 酵母双杂交分析 A：CiNPR4与CsTGA2和CsTGA6的酵母双杂交分析Yeast-two-hybrid assay of CiNPR4 with CsTGA2 and CsTGA6, respectively;B：CsTGA2和CsTGA6与空载pGBKT7的酵母双杂交分析Yeast-two-hybrid assay of CsTGA2 and CsTGA6p with the empty pGBKT7 vector, respectively;C：阳性对照（pGBKT7-53和pGADT7-Rec）和阴性对照（pGBKT7-Lam和pGADT7-Rec）的酵母双杂交分析Yeast-two-hybrid assay of positive (pGBKT7-53 and pGADT7-Rec) and negative (pGBKT7-Lam and pGADT7-Rec) controls;D：酵母双杂交阳性克隆的PCR验证Confirmation of the positive clones of CiNPR4 and CsTGA2 by PCR DDO：缺少色氨酸和亮氨酸的SD培养基 SD/-Trp and -Leu;QDO/X/ABA：缺少腺嘌呤和组氨酸,添加α-半乳糖苷酶和金担子素的DDO培养基DDO/-Ade/-His + X-α-Gal and aureobasidin A;M：DNA Ladder;1—6：阳性克隆Positive clone;-：水对照H2O control;+：CsTGA2（上）和CiNPR4（下）质粒CsTGA2 (upper) and CiNPR4 (below) plasmids

Fig. 3 Yeast-two-hybrid interaction assays