转CiNPR4基因柑橘抗溃疡病的机制解析

张婧芸,刘语诺,王兆昊,彭爱红,陈善春,何永睿

Analysis of Resistance Mechanism of CiNPR4 Transgenic Plants to Citrus Canker

ZHANG JingYun,LIU YuNuo,WANG ZhaoHao,PENG AiHong,CHEN ShanChun,HE YongRui

表1 本研究所用引物

Table 1 Primers used in this study

引物名称 Primer name 引物序列 Primer sequence (5′→3′) 用途 Usage CsPR1-f AAATGTGGGTGAATGAGAAAGC CsPR1的表达分析 Expression analysis of CsPR1 CsPR1-r ATTATTGTTGCACGTCACCTTG CsPDF1.2-f CAGTGGCAGAAGCAAAACAA CsPDF1.2的表达分析 Expression analysis of CsPDF1.2 CsPDF1.2-r CCGGGGAAGTCGTAGTGGC Actin-f CATCCCTCAGCACCTTCC 柑橘actin的表达分析 Expression analysis of citrus actin Actin-r CCAACCTTAGCACTTCTCC CiNPR4-f CATGGAGGCCGAATTATGGTTGAGAAGGCTCTCGTGG CiNPR4的cDNA扩增 Amplification of cDNA from CiNPR4 CiNPR4-r GGATCCCCGGGAATTTTAATTGCTCGCTGCCGAGG CsTGA2-f GCCATGGAGGCCAGTGAATTCATGGAGAATGCTGTTGACCT CsTGA2的cDNA扩增 Amplification of cDNA from CsTGA2 CsTGA2-r GCAGCTCGAGCTCGATGGATCCTCACTCCCGTGGCCGGGCCA CsTGA6-f GCCATGGAGGCCAGTGAATTCATGCCGAGCTTTGATTCTC CsTGA6的cDNA扩增 Amplification of cDNA from CsTGA6 CsTGA6-r GCAGCTCGAGCTCGATGGATCCTCACTCTCTTGGGCGGGC