利用CRISPR/Cas9基因编辑技术构建水稻ospin9突变体

吴世洋,杨晓祎,张艳雯,侯典云,胥华伟

Generation of ospin9 Mutants in Rice by CRISPR/Cas9 Genome Editing Technology

WU ShiYang,YANG XiaoYi,ZHANG YanWen,HOU DianYun,XU HuaWei

图3 OsPIN9转基因植株的鉴定及纯合突变体的筛选 A：PCR扩增HPT片段鉴定OsPIN9编辑植株;B：纯合突变体的筛选

Fig. 3 Identification of the transgenic lines and screening of ospin9 homozygous mutants by sequencing A: Identification of the transgenic lines by PCR amplification of HPT; B: Screening of the homozygous ospin9 mutants by sequencing