利用CRISPR/Cas9基因编辑技术构建水稻ospin9突变体

吴世洋,杨晓祎,张艳雯,侯典云,胥华伟

Generation of ospin9 Mutants in Rice by CRISPR/Cas9 Genome Editing Technology

WU ShiYang,YANG XiaoYi,ZHANG YanWen,HOU DianYun,XU HuaWei

图2 OsPIN9 gRNA靶位点示意图及阳性克隆筛选 A：OsPIN9靶位点示意图;B：PCR扩增OsU6启动子筛选阳性克隆

Fig. 2 Schematic diagram of OsPIN9 target site and screening for positive clones A: Schematic diagram of OsPIN9 target site; B: Screening for positive clones by PCR amplification of OsU6 promoter