苦荞WOX家族全基因组鉴定及响应愈伤诱导率表达分析

侯思宇,王欣芳,杜伟,冯晋华,韩渊怀,李红英,刘龙龙,孙朝霞

Genome-Wide Identification of WOX Family and Expression Analysis of Callus Induction Rate in Tartary Buckwheat

HOU SiYu,WANG XinFang,DU Wei,FENG JinHua,HAN YuanHuai,LI HongYing,LIU LongLong,SUN ZhaoXia

表2 实时荧光定量PCR引物序列

Table 2 The primer sequence for qRT-PCR

引物名称 Primer name 正向引物 Forward sequences (5'-3') 反向引物 Revered sequences (5'-3') 扩增长度 Amplification length (bp) FtWOX1 CAGGCGGTGTCTTCTCGATT AACTGACCGGAAGATGGGTG 94 FtWOX2 GATGAAGGACGACGAGCCAG AGAACTCGGTAGGGTTGCAG 137 FtWOX3 CGAGGGGAACGATAAGGTGG GACTCAACTTTGCCATGCTCC 142 FtWOX4 CTCAAGCGCCTTACTCCCTC GCTTTCAAACGCCCTACTCG 102 FtWOX5 AGGACCTGCAAATGGGAAGC TCTGAGACGGCTGCTACTCT 138 FtWOX6 AAGGTGCTCTAATGGCCGC TTGGTCAACGCTTAAACGCC 137 FtWOX7 TTCAAGTCCAAGCCCTGCAA CTCCGCTGTCGATCCATCTC 108 FtWOX8 ACACGACTCTCTCCTGCTTG TGTCGTTGGACTGTTGTGGT 142 FtWOX9 AGTGGACAGTTCGTTGCTGA GGACTCCTGACCGTCGTAAC 133 FtWOX10 ACTACATTGGCACCTGGTCG CCACTAAGCTGCCATCCTCC 124 FtWOX11 AGGCGTGCTAGATTGAAGAGG CTTGTGGCTGGGAATCCTCG 124 FtWOX12 AGAGGACCACCATAGACTCG ACTGAACCGGACCCTCATTC 98 FtWOX13 ACCTTCCAATCAGTTCGCGG ACTTCAAGTTGCTCGTCGGT 117 FtWOX14 GGTGGAACCCTACGCAAGAG CTGAGTTGCGTCGTGATGTG 106 FtWOX15 GGTAGTCCGACTTGAAGGGC CAACCGCCAACTGCATTCTC 111 FtWOX16 AGGCGTGCTAGATTGAAGAGG CTTGTGGCTGGGAATCCTCG 124 FtWOX17 CCTCGAGAAGAGCTTCGACA AACCAGATAGCAACCTGCCG 102 FtWOX18 ACCAAGCTTGAACCACGTCA AGCTGCTTCGACTTCCATCG 113 FtWOX19 ACGGAGATGGAGTGCGAGTA GTAGAGCTCGGCACAGTCAA 128 FtWOX20 TCTCACAGAGCAGAATCGCTG GTCCTTGAATGGCAGGAGGC 129 FtWOX21 GCGGCATTGCGTCATCTAAG TTCAACCTCTGGCTGAGTGC 107 FtWOX22 TCTTGCCCGTTGGATCACTC GCAACACAAGATGGCATCCG 127 FtWOX23 CGTGATTGCCGAGCTGTAGA AGCCGATGCCAAAGTCGTAA 108 FtWOX24 TGAGGAGAAGTCTCCAGGGT CCGGTTCAGGCCTCATCATA 104 FtWOX25 CGTGAACAGGAGATGCCGAT TACTGAACTTGCGTCGGTGT 120 FtWOX26 TGTGAAGAAAGCTCCCTCGG TTCTGTATTTGCTGCGCCGT 111 FtWOX27 CATACGATGACTCTCCAGGGC ATCTAGCGGCGGATGTTCG 116 FtWOX28 TCAGCGATGAGCAGATCAGG CATCTGGCTCGTCGGTTCTG 145 FtWOX29 AAGCTTGCTCGTGAGCTTGG ATGGAGACGGCATCGAACTC 134 FtWOX30 TACGCTCCGGTTGACATTCC AGGCAACAGAGAGAAGCGAC 130 FtHis ATCGACTGGAGGAAAGGCTC GCGGTATCTGTGGGACTTCT 106