荔枝果皮BPox的分离纯化及其在果实成熟过程中的表达

郭志雄,孙冷雪,郑嘉敏,蔡灿军,王蓓,李开拓,潘腾飞,佘文琴,陈桂信,潘东明

Purification, Characterization and Expression of Ionically Bound Peroxidase in Litchi Pericarp during Coloration and Maturation of Fruit

GUO ZhiXiong,SUN LengXue,ZHENG JiaMin,CAI CanJun,WANG Bei,LI KaiTuo,PAN TengFei,SHE WenQin,CHEN GuiXin,PAN DongMing

表2 金属离子及抑制剂对BPox-2/3活性的影响

Table 2 Effects of metal ions and inhibitors on the activity of BPox-2/3

抑制剂 Inhibitor 终浓度 Concentration (mmol∙L-1) BPox-2相对活性 Relative activity of BPox-2 BPox-3相对活性 Relative activity of BPox-3 CuSO4 0 1 1 0.1 1.05±0.016 1.18±0.008 1 1.05±0.003 1.06±0.01 MnSO4 0.1 0.92±0.009 0.87±0.019 1 0.74±0.013 0.78±0.01 CaCl2 0.1 0.98±0.010 0.99±0.002 1 0.88±0.011 0.89±0.03 FeCl3 0.1 0.92±0.009 0.91±0.020 1 0.77±0.026 0.85±0.03 EDTA 0.1 0.93±0.007 0.95±0.005 1 0.89±0.06 0.93±0.03 L-Cysteine 0.1 0.15±0.005 0.22±0.001 1 0.019±0 0.058±0 DTT 0.1 0.03±0.003 0 1 0 0 ASA 0.1 0 0 1 0 0