荔枝果皮BPox的分离纯化及其在果实成熟过程中的表达

郭志雄,孙冷雪,郑嘉敏,蔡灿军,王蓓,李开拓,潘腾飞,佘文琴,陈桂信,潘东明

Purification, Characterization and Expression of Ionically Bound Peroxidase in Litchi Pericarp during Coloration and Maturation of Fruit

GUO ZhiXiong,SUN LengXue,ZHENG JiaMin,CAI CanJun,WANG Bei,LI KaiTuo,PAN TengFei,SHE WenQin,CHEN GuiXin,PAN DongMing

表1 BPox-2/3对不同底物的比活力

Table 1 Specific activity of BPox-2/3 for different substrates

底物 Substrate	波长 Wavelength (nm)	BPox比活力 Specific activity of BPox (×10⁵ U∙mg^-1)
底物 Substrate	波长 Wavelength (nm)	BPox-2	BPox-3
愈创木酚 Guaiacol	470	116.03±1.41	31.38±0.45
(-)-表儿茶素 (-)-Epicatechin	440	61.66±1.87	62.71±0.40
4-甲基邻苯二酚4-Methylcatechol	420	16.85±0.21	3.93±0.07
焦性没食子酸 Pyrogallol	420	11.75±0.13	3.61±0.17
邻苯二酚 Catechol	420	8.37±0.19	3.45±0.34
没食子酸 Gallic acid	420	3.82±0.09	0.88±0.16
氯原酸 Chlorogenic acid	420	3.09±0.29	0.84±0.01
4-甲氧基酚 4-Methoxyphenol	420	0.88±0.09	0.53±0.18
对苯二酚 Hydroquinone	420	0.44±0.09	0.48±0.07
间苯二酚 m-Dihydroxybenzene	420	0	0
藜芦醇 Veratryl alcohol	420	0	0
苯甲醇 Benzylalcohol	310	0	0