苹果叶片不定芽再生过程的差异表达基因鉴定与分析

刘锴,何闪闪,张彩霞,张利义,卞书迅,袁高鹏,李武兴,康立群,丛佩华,韩晓蕾

Identification and Analysis of Differentially Expressed Genes in Adventitious Shoot Regeneration in Leaves of Apple

LIU Kai,HE ShanShan,ZHANG CaiXia,ZHANG LiYi,BIAN ShuXun,YUAN GaoPeng,LI WuXing,KANG LiQun,CONG PeiHua,HAN XiaoLei

表1 差异表达基因的定量引物验证

Table 1 Primers for quantitative validation of differentially expressed genes (DEGs)

基因ID Gene ID 基因名称 Gene name 正向引物 Forward primer 反向引物 Reverse primer MD02G1100200 SAUR72 AATTTCAGGAGACCTCCGCC CGTTGATCGTGTCGTCGTCT MD10G1121700 LAX2 AACACAACTTCCGCTCCTCC CCGGGCGTGGAGTAATCAAA MD05G1052100 SAUR14 TTCCGTCTTCCGGCTGTAAT TCTCGCCAACATAAACCGCA MD15G1208300 CKX3 ATACAACCCAGCAGCCGTTT CCTCTGGTGGAATGACCGTG MD01G1164700 MSD1 GCGCTCCGATTAGGTCTTCA TGGATCTGCATGATCTCGCC MD03G1014400 PA2 ACGGAAGCGGAGATACGTTG CTGATCGGTCTGGAGAAGCC MD10G1103500 LBD38 CCGTTTTGTTGTTGCCGAGT CAACGCAATGCAAGAGTGCT MD17G1073700 RAV2 TTGACCAAGGGATGGACACG TGAACCGCAGACTATCGACG MD13G1252700 PLT2 CCTCAGTTTGCTACTGCCGA AACCACGGAGGAAACTAGCG MD03G1118500 IFL TGCGGCAGTACCCATTGATT AGGCTTCCACATGACAGCAA MD08G1077100 ANT AATCCGTCCTCGTTGGTGAC CCGCAGAGGTTGGACTTACA MD01G1162100 PRX52 GCCTTGCAAGATGCACAACA AGGTTGTCGTCATTTCCGCT