苹果黑腐皮壳菌CAP超家族蛋白基因鉴定及毒性功能分析

王程利,尹志远,聂嘉俊,林永辉,黄丽丽

Identification and Virulence Analysis of CAP Superfamily Genes in Valsa mali

WANG ChengLi,YIN ZhiYuan,NIE JiaJun,LIN YongHui,HUANG LiLi

图6 基因敲除突变体及回补菌株PCR检测 野生型WT：03-8;Marker：DL2000;1—4：分别指各基因的4对检测引物（5F/6R、7F/8R、9F/10R和Neo-F/R）的PCR产物检测 PCR amplification product detection of 5F/6R, 7F/8R, 9F/10R and Neo-F/R for each gene。A：ΔVmPR1a-7和ΔVmPR1a-23突变体及回补菌株PCR检测 PCR amplification product detection of ΔVmPR1a-7, ΔVmPR1a-23 and complementation strain;引物VmPR1a-5F/6R检测回补菌株ΔVmPR1a/C The complementation strain ΔVmPR1a/C was detected by primer VmPR1a-5F/6R。B：ΔVmPR1b-20和ΔVmPR1b-31突变体PCR检测 PCR amplification product detection of ΔVmPR1b-20 and ΔVmPR1b-31。C：ΔVmPR1c-26和ΔVmPR1c-40突变体及回补菌株PCR检测 PCR amplification product detection of ΔVmPR1c-26, ΔVmPR1c-40 and complementation strain;引物VmPR1c-5F/6R检测回补菌株ΔVmPR1c/C The complementation strain ΔVmPR1c/C was detected by primer VmPR1c-5F/6R

Fig. 6 PCR detection of mutants and complementation strains