假禾谷镰孢转录因子FpAPSES的鉴定与功能分析

赵静雅,夏荟清,彭梦雅,凡卓,殷悦,徐赛博,张楠,陈文波,陈琳琳

Identification and Functional Analysis of Transcription Factors FpAPSES in Fusarium pseudograminearum

ZHAO JingYa,XIA HuiQing,PENG MengYa,FAN Zhuo,YIN Yue,XU SaiBo,ZHANG Nan,CHEN WenBo,CHEN LinLin

表1 本研究中所用的引物

Table 1 Primers used in the study

引物Primer 引物序列Primer sequence (5′-3′) FpAPSES1-F1 GTGGGTCTCATGATGATTC FpAPSES4-F1 GAGTCTTCAACCCCTCTC FpAPSES1-R1 CAATATCATCTTCTGTCGACGTTGAAAGATTGTTGAGATG FpAPSES4-R1 CAATATCATCTTCTGTCGACGTTGGCGACTAAGATGAAGC FpAPSES1-F2 ATAGAGTAGATGCCGACCGCGGGTTCTACCGGCACTCACCTCTC FpAPSES4-F2 ATAGAGTAGATGCCGACCGCGGGTTCTTAACATCATTGGCCTATC FpAPSES1-R2 CATACAAACATGCTGCCTCTC FpAPSES4-R2 CAATTGTCCGGTGCTGTC YG-F GATGTAGGAGGGCGTGGATATGTCCT HY-R GTATTGACCGATTCCTTGCGGTCCGAA HYG-F GTCGACAGAAGATGATATTG HYG-R GAACCCGCGGTCGGCATCTACTCTAT FpAPSES1-F3 CATTAGGCTCTTCGGGTGTG FpAPSES4-F3 CACACCAACCCAATCCCTC FpAPSES1-R3 CATACGAGACTCTACAACAG FpAPSES4-R3 GTTCTGGTTGCTGGTCCTG FpAPSES1-G1 CAACGACTCATGGCTCAATG FpAPSES4-G1 CATGCATATTCAGAACCCAG FpAPSES1-G2 GTAAGGGAGAGGTTCAAGAG FpAPSES4-G2 GTGCTGTGTGATCCTCTG H1R GCTGATCTGACCAGTTGC H1F GTCGATGCGACGCAATCGT H2F TTCCTCCCTTTATTTCAGATTCAA H2R ATGTTGGCGACCTCGTATTGG TEF1a-RTF TCACCACTGAAGTCAAGTCC TEF1a-RTR ACCAGCGACGTTACCACGTC FpAPSES1-RTF AACGTGGATATGTATGCGGC FpAPSES4-RTF GCTATGGGTGATTTGGATGTCG FpAPSES1-RTR TCGACATCTG GAGACATCTC FpAPSES4-RTR GTTCTTTCATGACCATAGG