二花脸猪linc-NORFA核心启动子鉴定与转录调控分析

杜星,曾强,刘禄,李琦琦,杨柳,潘增祥,李齐发

Identification of the Core Promoter of Linc-NORFA and Its Transcriptional Regulation in Erhualian Pig

DU Xing,ZENG Qiang,LIU Lu,LI QiQi,YANG Liu,PAN ZengXiang,LI QiFa

表1 本试验所用引物

Table 1 Primers used in this study

引物名称 Primer name 基因 Gene 引物序列 Sequence (5′-3′) 退火温度 Tm (℃) 大小 Size (bp) 用途 Usage P1 linc-NORFA F: TGGTCCAGGAGGGCAAGC 62.3 637 PCR扩增 PCR Amplification R: CCCCGTTTGCGTGCTTGT P2 linc-NORFA F: GGGTTTGTGAGTCCAAAGCAAG 61.9 796 R: GGTTTCCACGTCCCTCGGTAT P3 linc-NORFA F: TTCCAGAGCCTCCAAAGTGA 58.1 730 R: CCTTTGGGAGACCAGCACA pNORFA-1F linc-NORFA CCAGCATCGCGTCGTCTTT 61.8 280 载体构建 Vector Construction pNORFA-2F linc-NORFA TTCCAGAGCCTCCAAAGTGACT 58.8 613 pNORFA-3F linc-NORFA AGGCTGTGCCCAGCGCCTG 59.6 917 pNORFA-4F linc-NORFA GGGTTTGTGAGTCCAAAGCAA 60.2 1232 pNORFA-5F linc-NORFA GGTCGTCATGGAATATGCTCG 60.5 1771 pNORFA-R linc-NORFA CCTCCCAATACGATCTACTTCCTG - - NORFA-RT linc-NORFA F: ATCCGTTTAGGTCCGTTAGAGC 60.7 117 qRT-PCR R: GGAGGCTGAGTTTGCCACAA FOXO1-RT FOXO1 F: GGTCAAGAGCGTGCCCTACT 60.3 173 R: TCCCACTCTTGCCTCCCTCT GAPDH-RT GAPDH F: GGACTCATGACCACGGTCCAT 60.0 220 R: TCAGATCCACAACCGACACGT FBE-1 linc-NORFA F:ACCTGTAATCCTGCTGGTGAGA 58.0 258 ChIP R:CCACCCACAGGTCTTAATCCA FBE-X linc-NORFA F:ATATGCTCGGTCCTGTGGC 60.0 328 R:CCACCTGTCAACCATCCCT