类胡萝卜素裂解双加氧酶基因AgCCD4调控芹菜不同组织的着色

王昊,尹莲,刘洁霞,贾丽丽,丁旭,沈迪,冯凯,徐志胜,熊爱生

The Carotenoid Cleavage Dioxygenases Gene AgCCD4 Regulates the Pigmentation of Celery Tissues with Different Colors

WANG Hao,YIN Lian,LIU JieXia,JIA LiLi,DING Xu,SHEN Di,FENG Kai,XU ZhiSheng,XIONG AiSheng

表2 芹菜与其他植物CCD4蛋白氨基酸组成成分及理化性质分析

Table 2 Amino acid composition and physicochemical properties of CCD4 in celery and other plants

植物 Plant 氨基酸数Number of amino acid 理论分子质量 Relative molecular mass (kD) 等电点 pI 氨基酸比例 Ratio of amino acid (%) 总平均疏水性Grand average of hydrophobicity 脂肪族Aliphatic 芳香族Aromatic 酸性Positive 碱性Negative 芹菜 Apium graveolens 赛雪 Saixue 592 65.07 6.03 19 10 12 11 -0.269 津南实芹 Jinnan Shiqin 592 65.12 5.95 19 10 12 11 -0.297 胡萝卜 Daucus carota 588 64.68 6.21 19 10 12 11 -0.284 哥伦比亚锦葵 Herrania umbratica 606 66.72 6.55 21 9 12 11 -0.224 矮牵牛 Petunia hybrida 603 66.01 6.25 20 10 12 11 -0.223 烟草 Nicotiana tabacum 601 65.99 7.16 20 10 13 11 -0.218 枸杞 Lycium chinense 599 65.71 6.34 20 9 13 11 -0.252 欧洲甜樱桃 Prunus avium 597 65.70 6.21 20 10 12 11 -0.238 银白杨 Populus alba 611 66.64 6.26 21 9 12 11 -0.189 黄连木 Pistacia vera 616 67.69 6.44 21 9 12 10 -0.176 甘薯 Ipomoea batatas 594 67.66 5.76 21 9 12 11 -0.171 中华辣椒 Capsicum chinense 603 66.04 6.34 21 9 12 11 -0.248 杨梅 Morella rubra 623 68.40 6.72 20 9 13 11 -0.259 向日葵 Helianthus annuus 592 64.80 5.70 20 10 12 12 -0.155 莴苣 Lactuca sativa 592 65.36 6.05 19 10 13 11 -0.263 山杜鹃 Rhododendron kaempferi 600 65.65 6.17 20 10 12 11 -0.187 克莱门柚 Citrus clementina 603 66.45 6.87 21 9 13 11 -0.236 芜菁 Brassica rapa 595 65.60 6.19 21 9 13 12 -0.230 盐芥 Eutrema salsugineum 602 66.41 7.02 21 8 14 11 -0.255 蓖麻 Ricinus communis 618 68.07 6.85 21 9 12 11 -0.133