柑橘黄化脉明病毒RT-RPA检测方法的建立
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马志敏,许建建,段玉,王春庆,苏越,张琦,宾羽,周常勇,宋震
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Establishment of RT-RPA for Citrus Yellow Vein Clearing Virus (CYVCV) Detection
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MA ZhiMin,XU JianJian,DUAN Yu,WANG ChunQing,SU Yue,ZHANG Qi,BIN Yu,ZHOU ChangYong,SONG Zhen
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表2 不同柑橘品种的RT-RPA 及RT-PCR 检测
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Table 2 CYVCV detection using RT-RPA and RT-PCR for different citrus varieties
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品种
Variety | 样品株数
Number of samples | 阳性样品数（阳性率） Positive number (Positive rate) | | RT-RPA | RT-PCR | | 尤力克柠檬 Eureka lemon | 5 | 5 (100%) | 5 (100%) | | 纽荷尔脐橙 Newhall navel orange | 5 | 5 (100%) | 5 (100%) | | 塔罗科血橙 Tarocco | 5 | 5 (100%) | 5 (100%) | | 冰糖橘 Bingtangju | 5 | 5 (100%) | 5 (100%) | | 默科特橘 Murcott angerine | 5 | 5 (100%) | 5 (100%) | | 沙田柚 Shatianyou | 5 | 2 (40%) | 2 (40%) | | 强德勒柚 Chandler pummelo | 5 | 0 (0) | 0 (0) | | 沃柑 Orah | 5 | 5 (100%) | 5 (100%) | | 爱媛38 Ehime 38 hybrid citrus | 5 | 5 (100%) | 5 (100%) | | 总计 Total | 45 | 37 (82.2%) | 37 (82.2%) |
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