柑橘黄化脉明病毒RT-RPA检测方法的建立
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马志敏,许建建,段玉,王春庆,苏越,张琦,宾羽,周常勇,宋震
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Establishment of RT-RPA for Citrus Yellow Vein Clearing Virus (CYVCV) Detection
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MA ZhiMin,XU JianJian,DUAN Yu,WANG ChunQing,SU Yue,ZHANG Qi,BIN Yu,ZHOU ChangYong,SONG Zhen
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表1 本试验所用引物
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Table 1 Primers used in this study
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体系
System | 引物名称
Primer name | 引物序列
Primer sequence (5′-3′) | 引物序列位置
Primer sequence position | RT-RPA
| CY1-F | GTCACTAACT CCATAGCTAA CCCTAAACAGAC | 6561-6737 | | CY1-R | [biotin]-GTTGAGATCTTCGTTCGCTCCAATAATATCAG | | CY1-Probe | [6-FAM]CT ATCGGGAAAG CTTGGGTCCG CATCTTG[THF]AC ATCGATCCTG CCAAC-C3 Spacer | 6598-6644 | | CY2-F | AAAACCCACA ACAATGGGGT CTCTCCACCT GCC | 6462-6652 | | CY2-R | AAGAACACGTTGGCAGGATCGATGTTCAAGATGCG | | CY3-F | GTCTACCGACGACAACAAGGGCAAACAACC | 6287-6435 | | CY3-R | TACGCTTTTCGATGGGCTCTTGGTTTTCCT | | CY4-F | AACAAGGGCAAACAACCACTTCACCCGACA | 6300-6435 | | CY4-R | TACGCTTTTCGATGGGCTCTTGGTTTTCCT | | CY5-F | GCCCTAACGACACGACCCCGAAACCTATCC | 6337-6439 | | CY5-R | GTGATACGCTTTTCGATGGGCTCTTGGTTT | | RT-PCR | 614F | TACCGCAGCTATCCATTTCC | 6174-6785 | | 614R | GCAGAAATCCCGAACCACTA |
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