甘薯病毒病发生关键因素研究

赵付枚,王爽,田雨婷,乔奇,王永江,张德胜,张振臣

An Investigation into Key Factors Influencing the Occurrence of Virus Disease in Sweet Potato

ZHAO FuMei,WANG Shuang,TIAN YuTing,QIAO Qi,WANG YongJiang,ZHANG DeSheng,ZHANG ZhenChen

表1 引物序列及扩增片段大小

Table 1 Primer sequences used for PCR analysis

目标病毒 Target virus 引物名称 Primer 引物序列 Primer sequence (5′- 3′) 片段大小 Expected size (nt) Sweepoviruses SweepoCP-F1 ACTTCGAGACAGCTATCGTGC 645 SweepoCP-R1 ATAYACAGGATTRCTAGCATG SPFMV SPFMV-F1 TGGGGTTATGATGAACTTCTTC 400 SPFMV-R1 TTCTGGAATGRYTGCGGGTTG SPCSV SPCSV-F1 TGGGAAGAMGAGAYATGGAG 573 SPCSV-R1 GAGCGTTCCTTTTCATCATC CMV CMR3-N50 CGCAGGTGGTTAACGGT 697 CMR3-C30 CCA CACGGTAGAATCAAAT SPVG SPVG-F1 GCAGGAAAAACAGGAAACAC 792 SPVG-R1 ATACCTCGGCATGTATGCCTT SPLV SPLV-F1 AATGCCAAGGCTACAAGGAGT 553 SPLV-R1 CAAGTAGTGTGTGTATGTTCC SPV2 SPV2-F1 TGAACCAGTGCATAGATTGG 770 SPV2-R1 TTCACACCTTCATACCATGC SPVC SPVC-F1 GGACGAACAATGAAAGTTGG 600 SPVC-R1 CACTTTCCTGTAATCTGCTG