大丽轮枝菌木糖苷酶基因的鉴定及基于HIGS技术的功能分析
张小雪(
),孙天歌,张迎春,陈丽华,张新宇,李艳军(),孙杰
),孙天歌,张迎春,陈丽华,张新宇,李艳军(),孙杰
Identification of Xylosidase Genes from Verticillium dahliae and Functional Analysis Based on HIGS Technology
ZHANG XiaoXue(),SUN TianGe,ZHANG YingChun,CHEN LiHua,ZHANG XinYu,LI YanJun(),SUN Jie
),SUN TianGe,ZHANG YingChun,CHEN LiHua,ZHANG XinYu,LI YanJun(),SUN Jie
图8. HIGS处理棉株的大丽轮枝菌恢复试验和相对含量检测
A：接种大丽轮枝菌14 d后pTRV2-00和pTRV2-VdxyL3 处理植株茎秆中病原菌相对含量测定;B：接种大丽轮枝菌14 d后pTRV2-00和pTRV2-VdxyL3处理植株茎秆大丽轮枝菌恢复培养。将茎秆置于PDA培养基,25℃培养箱中培养7 d后拍照
Fig. 8. Recovery experiment and relative content detection of V. dahliae in cotton plants treated with HIGS
A:Quantification of the relative fungal biomass in stems of the pTRV2-00 and pTRV2-VdxyL3 treated plants at 14 dpi;B:Fungal isolation in the stem sections from pTRV2-00 and pTRV2-VdxyL3 treated plants at 14 dpi. Stems were plated on PDA medium. Photos were taken at 7 dpi of culture at 25℃
