大丽轮枝菌木糖苷酶基因的鉴定及基于HIGS技术的功能分析
张小雪(
),孙天歌,张迎春,陈丽华,张新宇,李艳军(),孙杰
),孙天歌,张迎春,陈丽华,张新宇,李艳军(),孙杰
Identification of Xylosidase Genes from Verticillium dahliae and Functional Analysis Based on HIGS Technology
ZHANG XiaoXue(),SUN TianGe,ZHANG YingChun,CHEN LiHua,ZHANG XinYu,LI YanJun(),SUN Jie
),SUN TianGe,ZHANG YingChun,CHEN LiHua,ZHANG XinYu,LI YanJun(),SUN Jie
图6. HIGS沉默效果检测
A：注射pTRV2-GhCHLI 10 d后感病品种新陆早8号的表型;B：qRT-PCR 检测VdxyL3在pTRV2-00 和pTRV2-VdxyL3处理植株中的表达量。从接菌14 d后植株茎秆中提取棉花总RNA。Tubulin为内参基因
Fig. 6. Detection of HIGS silencing effect
A: Phenotypes of susceptible variety Xinluzao 8 after 10 days of injection with pTRV2-GhCHLI;B:qRT-PCR analysis of VdxyL3 expression in the pTRV2-00 and pTRV2-VdxyL3 treated plants. Total RNA was isolated from stems at 14 dpi. Tubulin was used as the control
