大丽轮枝菌木糖苷酶基因的鉴定及基于HIGS技术的功能分析

张小雪,孙天歌,张迎春,陈丽华,张新宇,李艳军,孙杰

Identification of Xylosidase Genes from Verticillium dahliae and Functional Analysis Based on HIGS Technology

ZHANG XiaoXue,SUN TianGe,ZHANG YingChun,CHEN LiHua,ZHANG XinYu,LI YanJun,SUN Jie

表2 大丽轮枝菌中木糖苷酶基因的鉴定

Table 2 Identification of xylosidase genes in V. dahliae

基因名称 Gene name 序列号 Sequence number 编码序列 Coding sequence (bp) 编码蛋白 Coding protein (aa) 分子量 Molecular weight (kD） 等电点 pI 基因的描述 Gene description 亚细胞定位预测 Subcellular location prediction VdxyL1 VDAG_01169 1786 558 62.33 5.16 β-木糖苷酶β-xylosidase 细胞膜外的蛋白质 Extracellular proteins VdxyL2 VDAG_02166 1804 571 65.15 4.97 木糖苷酶/阿拉伯糖苷酶 Xylosidase/arabinosidase 细胞膜外的蛋白质 Extracellular proteins VdxyL3 VDAG_01866 2544 834 90.97 4.67 木糖苷酶/阿拉伯糖苷酶 Xylosidase/arabinosidase 细胞膜外的蛋白质 Extracellular proteins VdxyL4 VDAG_04702 1089 335 38.78 5.31 α-木糖苷酶α-xylosidase 细胞质Cytoplasm VdxyL5 VDAG_07817 1611 512 56.13 5.04 β-木糖苷酶β-xylosidase 细胞膜外的蛋白质 Extracellular proteins VdxyL6 VDAG_09393 1943 609 67.13 4.88 β-木糖苷酶β-xylosidase 细胞膜外的蛋白质 Extracellular proteins VdxyL7 VDAG_03859 1881 572 62.66 5.89 β-葡糖苷酶/β-木糖苷酶 β-glucosidase/β-xylosidase 溶酶体Lysosome VdxyL8 VDAG_05579 2353 764 85.22 5.18 α-木糖苷酶α-xylosidase 溶酶体Lysosome VdxyL9 VDAG_06173 2020 488 54.44 5.96 β-木糖苷酶β-xylosidase 高尔基体Golgi apparatus VdxyL10 VDAG_09302 1614 537 60.70 5.68 β-木糖苷酶β-xylosidase 细胞质Cytoplasm VdxyL11 VDAG_00716 1841 533 59.45 5.28 木糖苷酶/阿拉伯糖苷酶 Xylosidase/arabinosidase 细胞核Cell nucleus VdxyL12 VDAG_02226 1995 664 76.09 5.88 α-木糖苷酶α-xylosidase 细胞质Cytoplasm VdxyL13 VDAG_03628 1461 400 44.70 5.78 木糖苷酶/阿拉伯糖苷酶 Xylosidase/arabinosidase 细胞膜外的蛋白质 Extracellular proteins