大丽轮枝菌木糖苷酶基因的鉴定及基于HIGS技术的功能分析

张小雪,孙天歌,张迎春,陈丽华,张新宇,李艳军,孙杰

Identification of Xylosidase Genes from Verticillium dahliae and Functional Analysis Based on HIGS Technology

ZHANG XiaoXue,SUN TianGe,ZHANG YingChun,CHEN LiHua,ZHANG XinYu,LI YanJun,SUN Jie

表1 引物序列

Table 1 Primer sequences

基因名称 Gene name 引物名称 Primer name 引物序列 Primer sequence (5′-3′) 用途 Purpose VdxyL3 (VDAG_01866) VdxyL3-F1 GGAATTCCTGGGCCTCTTCGCTCTATG PCR VdxyL3-R1 GGGTACCACAGCACTGAGACCAGCATC PCR VdxyL3-F CGGAAACATTCACCTCCCCA qRT-PCR VdxyL3-R GGCGTAAGCCTCGAAAGCAT qRT-PCR VdxyL4 (VDAG_04702) VdxyL4-F CAGCGCCATTGAAATCGAGG qRT-PCR VdxyL4-R GGATTATTCCAGAGAAACCC qRT-PCR VdxyL6 (VDAG_09393) VdxyL6-F CTCAACGCCAGCTTCTACGT qRT-PCR VdxyL6-R CGTCGGGATCGTAGCGTAAA qRT-PCR VdxyL8 (VDAG_05579) VdxyL8-F TAAGACCATTGAGAGCCGCG qRT-PCR VdxyL8-R AGTTAACGTCGTGCCCTTGT qRT-PCR VdxyL10 (VDAG_09302) VdxyL10-F CGCCAAACCCACTTCTCCTA qRT-PCR VdxyL10-R TAGGACGACTCGGAGCTCAT qRT-PCR VdxyL12 (VDAG_02226) VdxyL12-F CGATCTGCTGCTTGAGGAGT qRT-PCR VdxyL12-R AGGTCGAGGAATGGCTGTTG qRT-PCR Tubulin Tubulin-F TCCACCTTCGTCGGTAACTC qRT-PCR Tubulin-R GCCTCCTCCTCGTACTCCTC qRT-PCR Ve-ITS1 Ve-ITS1-F AAAGTTTTAATGGTTCGCTAAGA qRT-PCR ST-VE1 ST-VE1-R CTTGGTCATTTAGAGGAAGTAA qRT-PCR GhUBQ7 GhUBQ7-F GAAGGCATTCCACCTGACCAAC qRT-PCR GhUBQ7-R CTTGACCTTCTTCTTCTTGTGCTTG qRT-PCR