巴什拜羊不同发育阶段骨骼肌中miR-486靶基因的研究

张伟,王世银,高莉,杨力伟,邓双义,刘晓娜,石国庆,甘尚权

Investigation of miR-486 Target Genes in Skeletal Muscle of Bashbay Sheep in Different Development Periods

ZHANG Wei,WANG ShiYin,GAO Li,YANG LiWei,DENG ShuangYi,LIU XiaoNa,SHI GuoQing,GAN ShangQuan

图4 候选靶基因荧光素酶报告载体试验 A为候选靶基因片段插入双荧光素酶报告载体位置及4个候选靶基因野生型（WT）和突变型（Mut）靶位点序列,序列中带下划线的碱基为通过重叠PCR获得的突变碱基;B为插入野生型和突变型靶位点片段荧光素酶报告载体的荧光素酶相对活性检测结果,NC指miR-486的阴性对照（Negative control）

Fig. 4 Double luciferase reporter assay experiments A: the insert site of candidate target gene fragment in vector and the sequence of wild (WT) and mutant (Mut) type, the base with underline are mutated bases achieved by overlap extension PCR. B: the relative luciferase activity determined 48 h after transfection. Data are expressed as means ± SD of three independent experiments, NC is negative control of miR-486