巴什拜羊不同发育阶段骨骼肌中miR-486靶基因的研究

张伟,王世银,高莉,杨力伟,邓双义,刘晓娜,石国庆,甘尚权

Investigation of miR-486 Target Genes in Skeletal Muscle of Bashbay Sheep in Different Development Periods

ZHANG Wei,WANG ShiYin,GAO Li,YANG LiWei,DENG ShuangYi,LIU XiaoNa,SHI GuoQing,GAN ShangQuan

表2 荧光素酶报告载体构建引物

Table 2 The primers used to construct luciferase reporter vectors

引物 Primer 引物序列(5′-3′) Sequence of primers 片段大小Size (bp) 试验用途 Experiment PTENcF ccctcgagggCACCTTTCTTTAGCATGCTAC 262 载体构建 Vector construction PTENcR ccaagcttggGATAGCCTCCACATTTGTATG PTENmutF GAATCTGTATTGGGGTACcttcATGAACCTTCCACAACAT 262 靶位点突变 Mutate bind site PTENmutR ATGTTGTGGAAGGTTCATgaagGTACCCCAATACAGATTC Foxo1cF ccctcgagggGAGAAGCAGTCCAAAGATGTC 221 载体构建 Vector construction Foxo1cR ccaagcttggATGGTGTAGTGAGTTTGGCAC Foxo1mutF CGAAGACGCTTCCTGTACcttcTGTTTGCCCAGTGTTTGC 221 靶位点突变 Mutate bind site Foxo1mutR GCAAACACTGGGCAAACAgaagGTACAGGAAGCGTCTTCG IGF1RcF ccctcgagggGAGAATCCCAATGGATTGATC 237 载体构建 Vector construction IGF1RcR ccaagcttggGGATGAAGTTCTCATATGTCG IGF1RmutF GTGTCCAGACAGGAGTACcttcAGTATGGAGGAGCCAAGC 237 靶位点突变 Mutate bind site IGF1RmutR GCTTGGCTCCTCCATACTgaagGTACTCCTGTCTGGACAC PIK3R1cF ccctcgagggCTGAAGGCTAAATTCACAGTG 267 载体构建 Vector construction PIK3R1cR ccaagcttggTAACAGCCAAGTACTCTGTAC PIK3R1mutF GCTTTTAAAGAAATGTACcttcTGCCAGTTTGTCAAGTCG 267 靶位点突变 Mutate bind site PIK3R1mutR CGACTTGACAAACTGGCAgaagGTACATTTCTTTAAAAGC