巴什拜羊不同发育阶段骨骼肌中miR-486靶基因的研究

张伟,王世银,高莉,杨力伟,邓双义,刘晓娜,石国庆,甘尚权

Investigation of miR-486 Target Genes in Skeletal Muscle of Bashbay Sheep in Different Development Periods

ZHANG Wei,WANG ShiYin,GAO Li,YANG LiWei,DENG ShuangYi,LIU XiaoNa,SHI GuoQing,GAN ShangQuan

表1 10个候选靶基因qRT-PCR引物信息表

Table 1 qRT-PCR primer of 10 candidate target genes

基因 Gene 登录号* Transcript ID 引物名称 Primers name 引物序列(5′-3′) Sequence of primers 片段大小 Size (bp) PTEN ENSOART00000015407.1 PTENqF GTATTTGCAGTATAGAGCGTGC 165 PTENqR GGATTTGATGACTCCTCTACTG Foxo1 ENSBTAT00000061127.3 Foxo1qF CCACAGCAATGACGACTTCGAC 275 Foxo1qR GACTGGGTGGACACAGTCAATG Dock3 ENSOART00000008795.1 Dock3qF AATCAGCCAAGCCTTCAGCTAG 246 Dock3qR TCTGCTCCCAGTCCATCATGTC PAX7 ENSOART00000011524.1 PAX7qF GTTCGATTAGCCGAGTGCTCAG 274 PAX7qR GTAGATGTCTGGGTAGTGGGTC ARHGAP5 ENSOART00000006997.1 ARHG5qF GTCTATCAGAACCATGTACAGC 189 ARHG5qR CATACACCTCTTTGCTATCAGC OLFM4 ENSOART00000007293.1 OLFM4qF GACCATCTCCAAGAAGTTCGAG 159 OLFM4qR CTTGATCAGCTCAAAGTCCAGC IGF1R ENSOART00000010895.1 IGF1RqF GGAAGAGCTCGAGACTGAGTAC 159 IGF1RqR GACAAAGTTAGAGGCGCTGCAG PIK3R1 ENSOART00000006390.1 PIK3R1qF CATGGGGATTACACTCTTACAC 147 PIK3R1qR CTAGAGATTCATTCCGGTAGTG FBN1 ENSOART00000022901.1 FBN1qF GGATTTCACGTCACACGAGATG 208 FBN1qR ACACAACGCCCATTCATGCAGA PDCD4 ENSOART00000011353.1 PDCD4qF ATTGCTAGAGCTGTTGGAGATG 250 PDCD4qR TCAGCTTCAGAAATGTCTCCAG β-actin ENSOART00000003275.1 β-actin qF TGTGCGTGACATCAAGGAGAAG 177 β-actinqR AGGAAGGAAGGCTGGAAGAG