苹果LRR-RLK基因家族鉴定和表达分析

黄金凤,吕天星,王寻,王颖达,王冬梅,闫忠业,刘志

Genome-Wide Identification and Expression Pattern Analysis of LRR-RLK Gene Family in Apple

HUANG JinFeng,LÜ TianXing,WANG Xu,WANG YingDa,WANG DongMei,YAN ZhongYe,LIU Zhi

表1 实时荧光定量引物序列

Table 1 Primers sequences of real time fluorescent quantitative PCR

登录号 Gene number 上游引物 Forward primer (5′-3′) 下游引物 Reverse primer (5′-3′) MdActin TGACCGAATGAGCAAGGAAATTACT TACTCAGCTTTGGCAATCCACATC MD09G1153800 TCTTCTTGAGCCATCTCC CGTCGGTCAGTATGTTTT MD04G1150400 ACCGTTCATTGCTCCTTG GCGAGCCCTATTTCCATC MD03G1138500 GAAGCCAACAGACGAGAT GGATAATAAGCAAACCCT MD06G1170200 CCAGTGGACCCATCATTC GCTTGGAGCATTTCTTGTAT MD03G1036900 GGAAAGGAGGAGCAGATT CAGGACCCAACAGAACAT MD05G1061600 AGATGTCTACAGCTATGGGATA TGGTTAGTTCGCCTTTGA MD13G1108000 TTCCCATAGACAGCAAGA GTGGCATCCAAGTAAAGC MD05G1261100 GTCGAAACCAAGCTATTT TAGTTGAGGCGAACATTA MD00G1105400 GACTGATAGGTTGGCACTG GACCCTCTGACTATGTCG MD02G1071800 GAATCCATCATAGAAACT ATTAGCCATGTACTCGTA MD05G1065800 TTCAATACCTCCGCAACT AAATCCCACTCAGCAAAT MD15G1288500 GCCTTGAGGATGGCAGAT GTACCCTAACTTGAACAACGAC