fl(2)d单等位基因的敲除显著降低小菜蛾的生殖力和育性
李飞飞,王贝贝,赖颖芳,杨菲颖,尤民生,何玮毅

Knockout of Single Allele of fl(2)d Significantly Decreases the Fecundity and Fertility inPlutella xylostella
LI FeiFei,WANG BeiBei,LAI YingFang,YANG FeiYing,YOU MinSheng,HE WeiYi
图4 fl(2)d基因突变对小菜蛾产卵量、孵化率和性别比例的影响
A:突变品系的产卵量The fecundity of mutant strains。B:突变品系的孵化率The hatchability of mutant strains。WT:野生型成虫杂交Hybridization between wild-type adults;F-4:杂合雌性Δfl(2)d213-4成虫与野生型杂交Hybridization of heterozygous female adult of Δ fl(2)d213-4 with wild-type;M-4:杂合雄性Δfl(2)d213-4成虫与野生型杂交Hybridization of heterozygous male adult of Δ fl(2)d213-4 with wild-type;-4:杂合Δfl(2)d213-4成虫近交Inbreeding between heterozygous adults of Δ fl(2)d213-4;F-5:杂合雌性Δfl(2)d213-5成虫与野生型杂交Hybridization of heterozygous female adult of Δ fl(2)d213-5 with wild-type;M-5:杂合雄性Δfl(2)d213-5成虫与野生型杂交Hybridization of heterozygous male adult of Δ fl(2)d213-5 with wild-type;-5:杂合Δfl(2)d213-5成虫近交Inbreeding between heterozygous adults of Δ fl(2)d213-5;F-7:杂合雌性Δfl(2)d214-7成虫与野生型杂交Hybridization of heterozygous female adult of Δ fl(2)d214-7 with wild-type;M-7:杂合雄性Δfl(2)d214-7成虫与野生型杂交Hybridization of heterozygous male adult of Δ fl(2)d214-7 with wild-type;-7:杂合Δfl(2)d214-7成虫近交Inbreeding between heterozygous adults of Δ fl(2)d214-7。C:性别比例The sex ratios of mutant offspring;采用t检验进行差异显著性分析,**表示显著性水平P<0.01 Significant difference analysis was performed usingt test. Double asterisks indicate significant difference of P<0.01
Fig. 4 Effects of fl(2)dmutation on the fecundity, hatchability and sex ratio of P. xylostella