fl(2)d单等位基因的敲除显著降低小菜蛾的生殖力和育性

李飞飞,王贝贝,赖颖芳,杨菲颖,尤民生,何玮毅

Knockout of Single Allele of fl(2)d Significantly Decreases the Fecundity and Fertility inPlutella xylostella

LI FeiFei,WANG BeiBei,LAI YingFang,YANG FeiYing,YOU MinSheng,HE WeiYi

图3 小菜蛾fl(2)d基因的编辑 A：fl(2)d基因结构及sgRNA靶点示意图Schematic diagram of the fl(2)d gene structure and sgRNA target site。B：G0代野生型（上）和突变体（下）成虫fl(2)d测序峰图;红色框是sgRNA,蓝色框是PAM结构Sequencing chromatograms of fl(2)d of wild-type (top) and mutant (bottom) adults; The edited site is indicated by a red rectangle and the protospacer adjacent motif (PAM) sequence is showed by a blue rectangle。C：突变序列,虚线表示缺失的序列Mutant sequences, the dashed lines represent the deleted bases caused by CRISPR/Cas9

Fig. 3 Gene editing of fl(2)din P. xylostella