fl(2)d单等位基因的敲除显著降低小菜蛾的生殖力和育性

李飞飞,王贝贝,赖颖芳,杨菲颖,尤民生,何玮毅

Knockout of Single Allele of fl(2)d Significantly Decreases the Fecundity and Fertility inPlutella xylostella

LI FeiFei,WANG BeiBei,LAI YingFang,YANG FeiYing,YOU MinSheng,HE WeiYi

图2 小菜蛾fl(2)d克隆及表达模式 A：fl(2)d CDS序列的克隆CDS cloning of fl(2)d;M：DNA分子标准DNA marker。B：预测的fl(2)d蛋白质序列Predicted protein sequence of fl(2)d;红色字母：保守功能域Red letters indicate the conserved domain。C：fl(2)d在不同发育阶段的表达Expression of fl(2)d in different developmental stages;E：卵Egg;FL：雌幼虫Female larva;ML：雄幼虫Male larva;FP：雌蛹Female pupa;MP：雄蛹Male pupa;FA：雌成虫Female adult;MA：雄成虫Male adult;采用单因素方差分析法进行差异显著性检验,使用Tukey法进行多重比较,不同字母表示差异显著（P<0.05）Significant difference analysis was performed using one-way ANOVA followed by a Tukey’s HSD post hoc test. Different letters indicate significant difference atP<0.05 level。D：fl(2)d在成虫生殖腺的表达Expression offl(2)d in adult gonads;O：卵巢Ovary;T：精巢Testis;采用t检验进行差异显著性分析,**表示检验性水平P<0.01 Significant difference analysis was performed usingt test. Double asterisks indicate significant difference of P<0.01

Fig. 2 Cloning and expression profiling of fl(2)d in P. xylostella