fl(2)d单等位基因的敲除显著降低小菜蛾的生殖力和育性

李飞飞,王贝贝,赖颖芳,杨菲颖,尤民生,何玮毅

Knockout of Single Allele of fl(2)d Significantly Decreases the Fecundity and Fertility inPlutella xylostella

LI FeiFei,WANG BeiBei,LAI YingFang,YANG FeiYing,YOU MinSheng,HE WeiYi

表1 本研究所用引物序列

Table 1 Primer sequences used in this study

引物名称Primer name 序列Sequence (5′-3′) 用途Usage Fl(2)d-ORF-F CGAGGAGACAGAACGCCTT 克隆fl(2)d Cloning offl(2)d Fl(2)d-ORF-R GGTGACGGTGAGGGGTTC qPCR-Fl(2)d-F CGGAGCTCAAATCATCACACGC fl(2)d定量表达 qRT-PCR of fl(2)d qPCR-Fl(2)d-R GCCCAGGTCCTCATTCTCCT RPL32-F CAATCAGGCCAATTTACCGC 内参基因RPL32定量表达 qRT-PCR of reference RPL32 RPL32-R CTGGGTTTACGCCAGTTACG Fl(2)d-Mut-TF GTATAATCCAATGAAGGTATGACAG 突变检测 Mutation detection Fl(2)d-Mut-TR CCTACAGTGAAACCCGCAA CRISPR-F1 TAATACGACTCACTATAGGAAGCCTGGAAAAGGCGAAAGGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCC 合成sgRNA Synthesis of sgRNAs CRISPR-F2 TAATACGACTCACTATAGGAAGGCTAGCAGCTAAAGAACGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCC CRISPR-R AAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAA