铜绿丽金龟气味结合蛋白AcorOBP11的表达纯化及功能分析

秦健辉,李金桥,赵旭,李克斌,曹雅忠,尹姣

Expression, Purification and Functional Analysis of Odorant Binding Protein 11 (OBP11) in Anomala corpulenta

QIN JianHui,LI JinQiao,ZHAO Xu,LI KeBin,CAO YaZhong,YIN Jiao

图2 AcorOBP11蛋白诱导表达及纯化 M：蛋白质分子量标准物Protein molecular weight marker;1：未诱导的pET28a-AcorOBP11菌体 Non-induced pET28a-AcorOBP11 in Escherichia coli;2：经过IPTG诱导表达的pET28a-AcorOBP11-His目的蛋白 Expressed protein pET28a-AcorOBP11-His after induction by IPTG;3：经过镍柱纯化获得 pET28a-AcorOBP11蛋白Purified protein pET28a-AcorOBP11 through Ni-NTA column;4：经过重组肠激酶切除His标签后的pET28a-AcorOBP11蛋白 Purified protein pET28a-AcorOBP11 with His-tag cleaved by recombinant enterokinase

Fig. 2 Expression and purification of the AcorOBP11 protein