铜绿丽金龟气味结合蛋白AcorOBP11的表达纯化及功能分析

秦健辉,李金桥,赵旭,李克斌,曹雅忠,尹姣

Expression, Purification and Functional Analysis of Odorant Binding Protein 11 (OBP11) in Anomala corpulenta

QIN JianHui,LI JinQiao,ZHAO Xu,LI KeBin,CAO YaZhong,YIN Jiao

Table 1 Binding properties of AcorOBP11 with different ligands

气味配体 Ligand 结构式 Structural formula 分子式 Formula IC50(μmol·L-1) Ki (μmol·L-1) α-紫罗兰酮 α-Ionone C13H20O 25.36 22.78 异戊醛 3-Methylbutanal C5H10O 27.27 24.49 丙烯酸-2-乙基己酯 2-Ethylhexyl acrylate C11H20O2 27.96 25.11 乙酸龙脑酯 Bornyl acetate C12H20O2 29.35 26.36 柠檬烯 Cinene C10H16 30.39 27.29 反-2-己烯醛 Trans-2-hexenal C6H10O 32.54 29.23 2-乙基己醛 2-Ethylhexanal C8H16O 33.33 29.94 异戊酸 Isovaleric acid C5H10O2 33.53 30.12 4-羟基-4-甲基-2-戊酮 4-Hydroxy-4-methyl-2-pentanone C6H12O2 33.55 30.13 庚醛 Heptanal C7H14O 36.34 32.64 丁醛 Butyraldehyde C4H8O 38.42 34.51 苯乙酸乙酯 Ethyl phenylacetate C10H12O2 38.54 34.61 辛醛 Octanal C8H16O 38.87 34.91 α-松油醇 α-Terpineol C10H18O 41.44 37.22 水杨酸甲酯 Methyl salicylate C8H8O3 41.83 37.57 顺-3-己烯基丁酯 Cis-3-hexenyl buyrate C10H18O2 42.84 38.48 1-辛烯-3-醇 1-Octen-3-ol C8H16O 44.37 39.85 戊醛 Pentanal C5H10O 44.40 39.88 己二酸二异丁酯 Diisobutyl adipate C14H26O4 45.49 40.86 反-2-壬烯醛 Trans-2-nonenal C9H16O 45.55 40.91 苯甲酸甲酯 Methyl benzoate C8H8O2 46.01 41.32 反式-2-己烯-1-醇 Trans-2-hexen-1-ol C6H12O 47.72 42.86 芳樟醇 Linalool C10H18O 48.53 43.59 β-石竹烯 β-Caryophyllene C15H24 48.71 43.75 β-罗勒烯 β-Ocimene C10H16 48.76 43.79 甲基庚烯酮 6-Methyl-5-hepten-2-one C8H14O 49.02 44.03 3,4-二甲基苯乙酮 3,4-Dimethyl phenylketone C10H12O 49.58 44.53 乙酸叶醇酯 Cis-3-hexenyl acetate C8H14O2 50.95 45.76 苯甲醛 Benzaldehyde C7H6O 51.45 46.21 2-壬酮 2-Nonanone C9H18O 53.77 48.29 2-辛醇 2-Octanol C8H18O 54.59 49.03 异丁醛 Isobutyraldehyde C4H8O 59.34 53.30 壬醛 Nonanal C9H18O 70.65 63.45 己酸 Caproic acid C6H12O2 152.96 137.37 α-丁香酚 α-Eugenol C10H12O2 N N 法尼烯 Farnesene C15H24 N N 十二碳烯 Propylene tetramer C12H24 N N