新疆马铃薯甲虫对噻虫嗪的抗性监测及其细胞色素P450基因表达分析

石鑫,李莎,王志敏,付开赟,付文君,姜卫华

Resistance Monitoring to Thiamethoxam and Expression Analysis of Cytochrome P450 Genes in Leptinotarsa decemlineata from Xinjiang

SHI Xin,LI Sha,WANG ZhiMin,FU KaiYun,FU WenJun,JIANG WeiHua

表6 抗性种群中表达量上调的基因

Table 6 Up-regulated genes in the resistant population

基因名称Gene symbol 基因号 Gene ID log2FC P值 Pvalue CYP4C1类基因 P450 4C1-like 111509985 3.75 9.82E-17 CYP9e2类基因 P450 9e2-like 111511395 2.15 0.001 可能为CYP305a1类基因 Probable P450 305a1 111504977 1.85 0.449 酯酶E4类基因Esterase E4-like 111513709 2.41 0.051 酯酶B1类基因%2C转录本变种X1 Esterase B1-like%2C transcript variant X1 111502042 1.18 0.190 羧酸酯酶6类基因 Carboxylesterase-6-like 111514530 1.07 0.553 2-羟乙酰鞘氨1-β类半乳糖糖基转移酶基因 2-hydroxyacylsphingosine 1-beta-galactosyltransferase-like 111516632 1.69 0.444 2-羟乙酰鞘氨1-β类半乳糖糖基转移酶基因 2-hydroxyacylsphingosine 1-beta-galactosyltransferase-like 111512327 1.90 0.457 未鉴定的LOC111503457 Uncharacterized LOC111503457 111503457 1.52 0.543 尿苷二磷酸-葡萄糖基转移酶2B30类基因 UDP-glucuronosyltransferase 2B30-like 111513316 2.52 0.023 蛹表皮蛋白36类基因 Pupal cuticle protein 36-like 111501889 3.28 0.073 幼虫表皮蛋白A2B类基因 Larval cuticle protein A2B-like 111503150 1.16 0.173 内表皮结构糖蛋白SgAbd-8类基因 Endocuticle structural glycoprotein SgAbd-8-like 111517484 3.91 0.173 ABC转运蛋白跨膜区 ABC transporter transmembrane region novel.1120 7.53 6.85E-06 多药耐药相关蛋白1类基因 Multidrug resistance-associated protein 1-like 111516459 7.98 7.63E-06 ABC转运蛋白 ABC transporter novel.1273 7.35 0.003 ATP-结合盒G亚家族类基因1%2C 转录本变种X1 ATP-binding cassette sub-family G member 1-like%2C transcript variant X1 111510979 1.79 0.553 烟碱型乙酰胆碱受体α3亚基 α3 111512651 1.00 0.683